Signal distributions (below) and corrected hook curves (above) of Universal Human Reference RNA hybridized on HG133A and HG133plus2 GeneChips (raw data were taken from ). The probe sets are composed either according to Affymetrics default settings (left part and "Affy" in the other parts), or using different customized transcript definitions (see ref. ; version 10; middle and right part) based on the annotations of different resources: ENSEMBLE (ENSG, ENSE, ENST), REFSEQ (see text). The probe sets of the HG133plus2 array were split into two subgroups which are either represented on both chip-types ("A") or on the P-chip only ("notA"). See the legends within the figure. The respective number of probe sets per array is given within the parentheses. The dotted lines in the lower panel serve as guide for the eye to characterize the respective decay constants λ. The hooks in the left part and the "Affy" hooks in the other parts are shifted in vertical direction each to another for sake of clarity. The dotted curves in the upper panel are fits of the hook-equation. The essential parameters are given in Table 3.