Table 1 Geometrical parameters of the hook curve

Start point

Σ(0) ≈ Σ^start,

1.0 – 2.5

Non-specific signal

Δ(0) ≈ Δ^start

0.0 – 0.15

PM/MM-gain (N)

End point

Σ(∞),

3.5 – 4.8

Saturation signal

Δ(∞)

0

PM/MM-gain (as)

Width

β = Σ(∞)-Σ(0)

2.2 – 3.2

Measuring range, non- specific binding strength in logarithmic scale

asymptotic height

α

0.75 – 1.1

PM/MM-gain (S)

decay constant

λ

0.5 – 1.5

Decay rate of the density distribution of the Σ-values; this S/N-index characterizes the mean ratio of specific and non- specific binding (S/N- ratio) in the logarithmic scale.

Expression index

φ = (β - $\frac{1}{2}$Δ(0)) - λ ≈ β - λ

1.5 – 2.5

Mean specific signal in logarithmic scale