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Figure 1 | Algorithms for Molecular Biology

Figure 1

From: "Hook"-calibration of GeneChip-microarrays: Theory and algorithm

Figure 1

PM- and MM-probe intensities (part a), their log-difference (part b) and -sum (part c) as a function of the specific target concentration (S/N-ratio). The curves are calculated using the Langmuir-model (Eq. (1)). The difference-plot reveals the hybridization regimes of non-specific (N), mixed (mix) and specific (S) hybridization, of saturation (sat) and the asymptotic (as) range. The parameters α and β used in Eq. (10) specify the data range spanned by the log-difference and -sum.

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