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Figure 10 | Algorithms for Molecular Biology

Figure 10

From: "Hook"-calibration of GeneChip-microarrays: Theory and algorithm

Figure 10

„Desaturated” hook-curve: The corrected intensities (see Figure 7) are linearized using Eq. (32), transformed into Δ and Σ coordinates (Eq. (9)) and smoothed. After "linearization" the sat-range of the hook levels off into the asymptote of height α (Eq. (24)) which defines the PM/MM-gain upon duplexing (compare with Figure 8). The fit neglects the saturation terms in Eq. (10).

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