Figure 3

Western blots for selected 21 miRs and Septin7. The Septin7 expression levels were measured (left to right) for (1) control sample, (2) miR-127, (3) miR-182, (4) miR-412, (5) miR-19a, (6) miR-453, (7) miR-448, (8) miR-450, (9) miR-183, (10) miR-141, (11) miR-202, (12) miR-148, (13) miR-106b, (14) miR-134, (15) miR-106, (16) miR-144, (17) miR-151, (18) miR-384, (19) miR-101, (20) miR-142, (21) miR-129 and (22) miR-126. The position 1 is the control sample; positions 2 to 11 inclusive concern 10 miRs for which predictions were obtained either by both PicTar and HiMiTar (positions 2, 3, 5, 9, and 10) or only by HuMiTar while these MiR were not included in the PicTar's database (positions 4, 6, 7, 8, and 11); positions 12 to 22 concern 11 miRs which were predicted by HuMiTar and which were included in the PicTar's database. We note that our analysis lacks results on the mutant targets that would strengthen the claim that the activation results of up-regulation of the predicted miRs. Due to limited resources and since the goal of this work is to present a new in-silico prediction method rather than to investigate whether miRs can up-regulate translation, we note that our conclusions concerning the up-regulation should not be considered as the primary outcome of this work.