L. collosoma. The predicted most significant mutation for the spliced leader RNA from L. collosoma. (A) Wild-type folded structure along with its representing dot plot matrix. The computed RNAfold global minimum energy is dG = -10.7. (B) The mutated folded structure with the largest distance grade from DoPloCompare (DP) = 0.102. The largest RNAdistance grade was also recorded for this structure (Rdist) = 52. The computed RNAfold global minimum energy is dG = -8.1 kcals/mole.