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Table 4 r2 for isoform and gene expression levels inferred from 30 M single, respectively paired reads of length 25, simulated assuming geometric expression of gene isoforms with and without poly(A) tails.

From: Estimation of alternative splicing isoform frequencies from RNA-Seq data

Reads Poly(A) Isoform Expression Gene Expression
1 × 25 Yes 0.956 0.977
  No 0.970 0.982
2 × 25 Yes 0.972 0.990
  No 0.976 0.985