Figure 1
From: A simple data-adaptive probabilistic variant calling model
Accumulation and sources of errors in next generation sequencing reads. The identification of single nucleotide variations (green dashes) is complicated by various sources of error. PCR errors accumulate during the amplification and sequencing step. After fragmentation single fragments undergo several amplification cycles. Errors are introduced with a rate δ f (red dashes). Further errors are accumulated during sequencing (δ g ). During the alignment to the reference further mismatches and indels are introduced (δ a ).