From: Designing minimal microbial strains of desired functionality using a genetic algorithm

i
| Design objective | Fitness function \(F_i\) |
---|---|---|

1 | Ethanol production with minimal MCS size | \(w_{1} \min Y_{Etoh} + w_{3} (1 - \vert C \vert /n)\) |

2 | Substrate specific productivity with minimal MCS size | \(w_{2} \min \eta _{Etoh} + w_{3} (1 - \vert C \vert / n)\) |

3 | Growth coupled product yield with minimal MCS size and maximum number of surviving modes | \(w_{1} \min Y_{Etoh} \times w_{2} \max \eta _{Etoh} + w_{3} (1 - \vert C \vert / n) + w_{4} \vert \mathbf D ^C \vert / \vert \mathbf E \vert\) |