Estimation of genetic diversity in viral populations from next generation sequencing data with extremely deep coverage

Table 1 Summary of sequence data analyzed

Sequenced data	Reads	Nucl.	Mapped (%)	Time (hour)
HIV1—non-stimulated CD4/R5	1.11 × 10⁷	5.53 × 10⁸	91.81	34.88
HIV2—stimulated CD4/R5	1.04 × 10⁷	5.19 × 10⁸	90.84	34.18
HIV3—non-stimulated CD4/X4	1.10 × 10⁷	5.50 × 10⁸	91.22	29.05
HIV4—stimulated PBMC/X4	9.41 × 10⁶	4.71 × 10⁸	91.14	13.63
HIV5—stimulated PBMC/R5	1.14 × 10⁷	5.73 × 10⁸	90.49	24.09
HIV6—non-stimulated PBMC/X4	1.12 × 10⁷	5.62 × 10⁸	91.42	27.54
HIV7—non-stimulated PBMC/R5	1.19 × 10⁷	5.94 × 10⁸	93.27	15.34
HIV8—control (pNL4-3kfs)	1.01 × 10⁷	5.05 × 10⁸	93.10	30.14
Total	8.65 × 10⁷	4.33 × 10⁹	91.67	208.85

The first column lists all the experimental conditions and the control experiment that where sequenced, the second column (Reads) displays the number of reads sequenced in each condition, the third column (Nucl.) displays the number of nucleotides in each condition, the fourth column (Mapped) displays the percentage of reads that have been mapped and the fifth column (Time) displays the time elapsed in each mapping procedure

ISSN: 1748-7188