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Table 1 List of all unique nucleotide sequence (UNS) of interest in the current application

From: Using a constraint-based regression method for relative quantification of somatic mutations in pyrosequencing signals: a case for NRAS analysis

Variant name Unique nucleotide sequence Reverse complementary
WT AGCTGGACAAG CTTGTCCAGCT
NRAS \(c.181C>G\) (Q61E) AGCTGGAGAAG CTTCTCCAGCT
NRAS \(c.181C>A\) (Q61K) AGCTGGAAAAG CTTTTCCAGCT
NRAS \(c.182A>T\) (Q61L-1) AGCTGGACTAG CTAGTCCAGCT
NRAS \(c.182A>C\) (Q61P) AGCTGGACCAG CTGGTCCAGCT
NRAS \(c.182A>G\) (Q61R-1) AGCTGGACGAG CTCGTCCAGCT
NRAS \(c.182\_183AA>TG\) (Q61L-2) AGCTGGACTGG CCAGTCCAGCT
NRAS \(c.182\_183AA>GG\) (Q61R-2) AGCTGGACGGG CCCGTCCAGCT
NRAS \(c.183A>C\) (Q61H-1) AGCTGGACATG CATGTCCAGCT
NRAS \(c.183A>T\) (Q61H-2) AGCTGGACACG CGTGTCCAGCT
  1. Nucleotides of codon 61 are in bold characters