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Table 4 Detailed classification results on five human-captured mouse exomes from different mouse strains (\(2\times \) A/J, \(1\times \) BALB/c, \(2\times \) C57BL/6)

From: Fast lightweight accurate xenograft sorting

A/J-1

xengsort

xenome

XfR

Time

70 Cm

14 Wm

371 Cm

45 Wm

56 Cm

56 Wm

Fragmets

(%)

Fragmets

(%)

Fragmets

(%)

Mouse

46,648,014

(78.03)

45,759,814

(76.54)

  

Both

120,808

(0.20)

65,269

(0.11)

  

Human

12,813,583

(21.43)

12,500,844

(20.91)

6,315,955

(10.56)

Ambgs.

58,449

(0.10)

1,383,547

(2.31)

  

Neither

143,775

(0.24)

75,155

(0.13)

  

A/J-2

xengsort

xenome

XfR

Time

70 Cm

15 Wm

416 Cm

50 Wm

67 Cm

67 Cm

Fragmets

(%)

Fragmets

(%)

Fragmets

(%)

Mouse

60,255,189

(95.57)

59,135,489

(93.80)

  

Both

151,396

(0.24)

89,089

(0.14)

  

Human

2,301,384

(3.65)

2,271,131

(3.60)

1,718,545

(2.73)

Ambgs.

57,827

(0.09)

1,340,814

(2.13)

  

Neither

279,556

(0.44)

208,829

(0.33)

  

BALB/c

xengsort

xenome

XfR

Time

68 Cm

15 Wm

392 Cm

45 Wm

61 Cm

61 Wm

Mouse

62,235,960

(98.99)

61,274,277

(97.46)

  

Both

118,541

(0.19)

68,949

(0.11)

  

Human

342,908

(0.55)

348,154

(0.55)

285,556

(0.45)

Ambgs.

45,063

(0.07)

1,098,036

(1.65)

  

Neither

127,035

(0.20)

80,091

(0.13)

  

C57BL/6-1

xengsort

xenome

XfR

Time

72 Wm

14 Wm

359 Wm

44 Wm

58 Cm

58 Wm

Mouse

57,993,361

(98.93)

57,522,446

(98.13)

  

Both

118,984

(0.20)

74,325

(0.13)

  

Human

375,716

(0.64)

376,653

(0.64)

290,894

(0.50)

Ambgs.

27,731

(0.05)

571,542

(0.98)

  

Neither

103,895

(0.18)

74,721

(0.13)

  

C57BL/6-2

xengsort

xenome

XfR

Time

67 Cm

15 Wm

422 Cm

51 Wm

62 Cm

62 Wm

Mouse

62,384,448

(99.00)

61,941,783

(98.30)

  

Both

107,019

(0.17)

66,163

(0.10)

  

Human

189,536

(0.30)

208,149

(0.33)

132,535

(0.21)

Ambgs.

27,142

(0.04)

562,659

(0.89)

  

Neither

304,677

(0.48)

234,068

(0.37)

  
  1. Running times are reported both in CPU minutes (Cm), measuring CPU work, and wall clock minutes (Wm), measuring actual time spent. Times for XenofilteR (XfR) do not include alignment or BAM sorting time. Classification results report the number and percentage (in brackets) of fragments classified as mouse (correct), both human and mouse (likely correct), human (incorrect), ambiguous (no statement) and neither (likely incorrect). XenofilteR (XfR) only extracts human fragments and does not classify the remainder; so only the number of fragments classified as human are reported