Fig. 5

Consistency test of anchor-linked long-read overlaps to direct alignments of both reads on fruit fly. (a) Frequencies of shifted offsets (% of the longer read); changes up to 5% are tolerated; note logarithmic axis. (b) Frequencies of the percentage at which the direct alignment covers the overlap. A minimum of 75% is set for consistency. (c) Long read pairs where no direct alignment is possible tend to have shorter anchor-indicated overlaps. Connections that cannot be confirmed via direct alignments despite an expected overlap of at least 1 kbp are excluded