LazyB: fast and cheap genome assembly

Table 2 Assessment of different parameters to verify long-read overlaps and their impact on LazyB assembly quality on fruit fly

Varification parameters	Compl.[%]	#ctg	#MA
Direction	80.13	608	111
Direction + offset	80.08	622	103
Direction + offset + incomplete mapping	80.04	1263	121
No mapping	80.15	801	113

Overlaps are indicated by anchors and evaluated by pairwise long-read alignments. They are considered valid if: the relative direction suggested by the anchor matches that of the pairwise alignment (direction); the offset is sufficiently similar for both methods (offset); at least 75% of the overlap is found as direct alignment (incomplete mapping); the overlap indicated by the anchor is less than or equal to 1 kbp or a pairwise alignment is possible (no mapping). Column descriptions: completeness of the assembly, #ctg: number of contigs, #MA: number of mis-assemblies (breakpoints relative to the reference assembly)

ISSN: 1748-7188