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Fig. 1 | Algorithms for Molecular Biology

Fig. 1

From: Bayesian optimization with evolutionary and structure-based regularization for directed protein evolution

Fig. 1

Traditional, model-free approaches to directed evolution: (Top) The ‘single mutation walk’ approach to directed evolution. The library of variants is the union of k libraries created by performing saturation mutagenesis at a single location. The resulting library, therefore, has 20k variants. The library is screened to find the single variant that optimizes the measured trait. That variant is fixed and the procedure is repeated for the remaining \(k-1\) positions. (Bottom) The library of variants is created by performing saturation mutagenesis at k positions. The top variants are identified through screening. Those variants are randomly recombined to generate a second library, which is then screened to find the top design

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