- Review article
- Open Access

*A priori* assessment of data quality in molecular phylogenetics

- Bernhard Misof
^{1}, - Karen Meusemann
^{1, 2}, - Björn M von Reumont
^{1, 3}, - Patrick Kück
^{1}, - Sonja J Prohaska
^{4, 5}and - Peter F Stadler
^{5, 6, 7, 8, 9, 10}Email author

**9**:22

https://doi.org/10.1186/s13015-014-0022-4

© Misof et al.; licensee BioMed Central Ltd. 2014

**Received:**21 May 2014**Accepted:**24 July 2014**Published:**12 September 2014

## Abstract

Sets of sequence data used in phylogenetic analysis are often plagued by both random noise and systematic biases. Since the commonly used methods of phylogenetic reconstruction are designed to produce trees it is an important task to evaluate these trees *a posteriori*. Preferably, however, one would like to assess the suitability of the input data for phylogenetic analysis *a priori* and, if possible, obtain information on how to prune the data sets to improve the quality of phylogenetic reconstruction without introducing unwarranted biases. In the last few years several different approaches, algorithms, and software tools have been proposed for this purpose. Here we provide an overview of the state of the art and briefly discuss the most pressing open problems.

## Keywords

- Phylogenomics
- Tree-likeness
- Phylogenetic networks
- Multiple sequence alignments
- Quartets
- Biases

## Introduction

Ideally, the evolutionary process generates data that conform to an additive tree structure. This ideal, however, is rarely if ever reached in practice. A diversity of natural processes conspire with imperfect models and methods of data analysis to cause sometimes large deviations. An unavoidable confounding factor is noise, introduced by the stochastic nature of sequence evolution itself, leading to a degradation of the phylogenetic signal when divergence times become very large and when data sets are small. Systematic biases are introduced by deviations from tree-like evolution, such as recombination and lateral gene transfer, as well as by violations of the model assumptions on which the data analysis is based, such as parallel evolution.

Nearly all methods of molecular phylogenetics, furthermore, use sequence alignments to obtain estimates of the divergence between taxa. For the purpose of phylogenetic reconstruction, each column of a multiple sequence alignment (MSA) is a character. In other words, the the letters in a column are treated as if they have arisen from a common ancestral state. All the algorithms for computing MSAs, however, explicitly or implicitly optimize cost functions (such as a sum of pair score) that are unaware of the detailed phylogenetic structure of the data set. This optimization problems, furthermore, are NP hard [1],[2] and hence can be solved only with (heuristic) approximation algorithms. MSAs, thus, are necessarily only approximations to a perfect assignment of homologous sequence positions. As most alignment methods internally use a guide tree representing a rough estimate of the particular phylogeny to determine the order in which taxa are treated, MSAs incorporate an implicit phylogenetic assumption that can be biased relative to the unknown true phylogenetic tree.

At the current state of the art, these issues are unavoidable at least in the analysis of large data set, although for small examples it may be feasible to employ methods that concurrently estimate alignments and trees directly from unaligned sequence data [3],[4]. Even in these cases biases from non-treelike evolution and insufficient knowledge remain. This is in particular true for the mechanisms of in/del formation.

It is good practice in phylogenetic studies, therefore, to estimate the reliability of the phylogenetic reconstructions *a posteriori*. Most commonly, measures such as the bootstrap or jackknife support or parameters such as the *consistency index* or the *retention index*, see e.g. [5] are used. The latter estimate the prevalence of homoplastic characters relative to the reconstructed tree.

An alternative approach, which is less frequently employed in phylogenetic studies, is to investigate the data set for its information content and possible source of problems already *before* even starting to compute trees. In this chapter we briefly review the most promising approaches for *a priori* quality control, focusing on recent developments.

## Measures of tree-likeness

### Distance-based measures

Tree reconstruction based on uncorrected distances obtained from discrete characters can lead to incorrect trees. Effects such as long branch attraction [6] have long been known and continue to be discussed in the literature, see e.g. [7]. The corresponding distances often deviate from additivity as indicated by conflicting support for alternative trees, and hence indications for misleading signals can be obtained from measures of tree-likeness.

*d*on a finite set

*X*of taxa forms an additive tree if and only if every quartet (set of four taxa) has this property [8],[9]. It appears natural, therefore, to use quartets to measure tree-likeness of a data set. Among four taxa {

*A*,

*B*,

*C*,

*D*} there are six distances which can be grouped into three pairs:

*d*(

*A*,

*B*)+

*d*(

*C*,

*D*),

*d*(

*A*,

*C*)+

*d*(

*B*,

*D*), and

*d*(

*A*,

*D*)+

*d*(

*B*,

*C*). Ordering these three sums by magnitude, we obtain three parameters

*L*?

*M*?

*S*, from which in turn we derive two split lengths

*?*=(

*L*?

*S*)/2 and

*?*=(

*L*?

*M*)/2, see Figure 1A. The quadruple is a tree if and only if

*L*=

*M*, i.e.,

*?*=0.

*statistical geometry*[11],[12] is to consider quadruples first in terms of distances and then in terms of sequence patterns. Three types of quadruple geometries can be distinguished based on distances alone:

- 1.
*L*=*M*=*S*. In this case*?*=*?*=0, the quadruple is an ideal bundle. - 2.
*L*=*M*>*S*. In this case*?*>0 and*?*=0 so that the quadruple defines a single split. - 3.
*L*>*M*>*S*. In this generic case the data deviate from tree structure.

It can be shown that the ratio *?*/*?* approaches 0.5 for random sequences, i.e., complete loss of phylogenetic signal. Averaging the parameters *?* and *?* over all quadruples that can be formed from a data set thus provide already a good measure for its tree-likeness.

The *?-plots*[13] build upon statistical geometry and represent the tree-likeness *?*/*?* of quartets in terms of a histogram. For an individual taxon a measure for tree-likeness can be obtained by considering the tree-likeness of all quartets to which it belongs. As suggested e.g. in [13], removing taxa with poor individual tree-likeness can result in increased accuracy of tree estimation.

It is important to note that the assessment of distance measures is not necessarily sufficient. Perfectly tree-like distances can still support an incorrect tree in realistic examples. Instructive cases are discussed in detail in [14]. A possible source of such biases is in particular the use of an incorrect model for the transformation of character differences to distances. A more detailed picture is obtained when the alignment columns for a sequence quadruple are inspected.

### Character-based methods

Recording for each column of a given alignment of four sequences only which of the sequences have the same character states we distinguish 16 column types that fall into just five classes *k*: all equal (1), one triple (4), two pairs (3), one pair (6), and all different (1). For alphabets with less than 4 letters not all of these can be realized. Given a model of evolution it is then possible to compute the expected values of the numbers *d*_{
k
} of occurrences of columns of type *k*, 0?*k*?4, as a function of the divergence (number of substitution events per site). From these value one can then obtain refined parameters for tree- and bundle-likeness, see [11],[12] for more details.

An alternative approach is to interpret the 16 column types as support for one of the three possible unrooted trees. Each quadruple, thus, can be associated with a relative support (*p*_{1},*p*_{2},*p*_{3}) for the three geometries. Properly normalized these values can be plotted in simplicial coordinates, Figure 1B. This idea underlies the *Quartet Mapping* method [15] and its special case *Likelihood Mapping*[16], where the values of *p*_{
i
} are computed as maximum likelihood estimates for the probabilities of the three tree topologies. Quartet mapping can also be used to resolve the relationships between four groups *A*, *B*, *C*, and *D* that are *a priori* known to be monophyletic. A special case, in which *A*={*x*} is just a single sequence is the problem of assigning individual genes to paralog groups. This technique, implemented in the software tool quartmquartm, has been used successfully e.g. to analyze short PCR fragments of homeobox sequences [10],[17],[18]. Figure 1 deliberately shows an example of extremely information-poor data.

A generalization to five taxa has been attempted more recently [19]. Finding the best planar visualization of the space representing the 15 possible unrooted trees for a given set of input data is a rather difficult optimization problem. PentaPlotPentaPlot[20] uses a genetic algorithm for this purpose.

On a set *X* of *n* taxa there are 2^{n?1}?1 distinct splits, i.e., bipartitions of the taxa into exactly two non-empty sets. Weight vectors $\stackrel{?}{q}$ over the set of splits are related to pattern probability vectors $\stackrel{?}{p}$ assigning probabilities to characters by means of the Hadamard transform $\stackrel{?}{p}={\mathbf{H}}^{?1}exp\left[\phantom{\rule{0.3em}{0ex}}\mathbf{H}\stackrel{?}{q}\right]$, where vector exponentiation is interpreted component-wise [21], see [22] for a modern presentation. Intuitively, the Hadamard transformation accounts for multiple state changes of a given character and provides a direct link between observed character states and splits in the underlying data. This connection can be used assess tree-likeness of the data by analyzing the split-spectrum. In addition to a support value *q*_{
s
} for a given split an incompatibility score can be defined as the sum of the supports for all splits *s* that cannot occur together with *s* in the same tree. This information is conveniently summarized in so-called Lento-plots [23]. SpectronetSpectronet provides an implementation [24]. A related method summarizes the Hadamard weight spectrum into three categories: the splits supporting external and internal branches of the optimal tree as well as the splits contradicting this tree. Plotting the relative weights of these three categories in barycentric coordinates produces a treeness triangle [25], from which deviation from tree-likeness can be assessed visually.

## Alignment quality

Large evolutionary distances inevitably entail a large number of homoplastic sites. As most protein-coding genes show dramatic variations in substitution rates that are not uncorrelated across the sequence, this often leads to a patchwork pattern of phylogenetically informative and effectively randomized regions. Alignment errors accumulate in highly variable regions and may produce effectively homoplastic sites. Both simulation studies [26] and evaluations of real-life data [27] demonstrated that alignment errors can significantly change the outcome of phylogenetic analyses. There is no consensus in the literature, furthermore, how tolerant phylogenetic methods are to multiple substitutions [28]-[30].

Consequently, one may try to improve the accuracy of tree reconstruction by eliminating all putative homoplastic or otherwise corrupted sites. A simple approach towards this end is to exclude all third-codon positions of protein-coding sequences. Since the quality of tree reconstruction decreases with decreasing sequence length, it is important not to remove too many sites from an alignment, however. For example, while certain first- and second-codon positions may be essentially constant (and therefore phylogenetically useless) or hyper-variable (and hence even misleading), third-codon positions of protein-coding genes can well be informative and thus they should not be discarded outright [31]. Instead, one would like to distinguish clearly homoplastic or otherwise corrupted sites from putative phylogenetically informative sites so that they and no others can be excluded or down-weighted.

The complication with such an endeavor, however, is that, formally, homoplasy is defined relative to a given phylogenetic tree, the very object that molecular phylogenetics is attempting to derive from the alignment. Measures such as the consistency index (the minimum possible number character changes divided by the number of steps observed along the tree) thus cannot be computed prior to estimating the phylogenetic tree itself. Consequently, the *a priori* is a difficult problem since a useful method has to ensure that its approach to homoplasy detection does not implicitly presuppose a phylogenetic tree later to be derived from the same data.

Historically the first tool for removing suspicious parts of alignments was GblocksGblocks[32],[33], which selects blocks from an input alignment using a set of rules that mimic many researchers strategy in manually pruning alignments. User-defined parameters set cut-offs so that the retained regions do not contain large segments of contiguous non-conserved positions, are depleted in gap positions, and exhibit high levels of conservation of flanking positions. While intuitively plausible, these rules are not based in some underlying theory. Nevertheless, this approach can lead to better trees, which, surprisingly, often exhibit reduced bootstrap support, indicating that divergent and problematic alignment regions may lead, when present, to apparently better supported although, in fact, more biased topologies [33].

EST-based phylogenomic studies are in particular plagued by incomplete sequences and thus by missing data in MSAs. This can introduce surprisingly large biases and substantially compromised phylogenetic accuracy [34],[35]. As a remedy, reapreap[34] masks (i) alignment columns containing many gaps and/or highly diverse amino acids and (ii) sequences that either have little overlap with other sequences or appear to be systematically misaligned. The cutoffs used in reapreap were determined empirically to strike the best compromise between topological accuracy and sequence retention [34

## Noisy

The noisynoisy[36] method is based on the observation that distances derived from pairwise sequence comparisons give rise to fairly robust circular split systems [37]. Circular splits systems can be represented as a circular ordering of the taxa and are consistent with a large number of possible tree topologies [38],[39], namely all those that can be inscribed in the circularly ordered taxa without crossings of tree edges. The utility of circular orderings computed e.g. by the Neighbor-NetNeighbor-Net[40] or QnetQnet[41] algorithms for our purposes is that phylogenetically more closely related taxa are preferentially placed closer together in the cyclic ordering. Conversely, similar trees necessarily correspond to similar cyclic orderings. Thus, if a character, i.e., an alignment column, is phylogenetically useful, its character states will appear clustered along the cyclic ordering underlying any tree that is a reasonable approximation of the true phylogeny, independent of the details of the branching order in individual subtrees. In contrast, if a character is completely randomized, we will observe that character states are randomly arranged along the cycle.

For a given cyclic ordering *?*, the amount of clustering in alignment column *i* is conveniently quantified as the number *?*(*?*,*i*) of break points, i.e., adjacent distinct character states. For constant alignment columns *?*(*?*,*i*)=0, for non-constant sites we have *?*(*?*,*i*)?2. This number has to be compared with the numbers expected for a random permutation of the letters observed in alignment column *i*. This background distribution is easily generated by means of shuffling, i.e., by replacing *?* with a random permutation *?*^{?} drawn from a uniform distribution. We then measure the fraction *q*(*?*,*i*) of sampled random permutations with *?*(*?*^{?},*i*)>*?*(*?*,*i*). The value of *q*(*?*,*i*) is thus an estimate for the probability that the column *i* is *not* randomized. The noisynoisy program removes all alignment columns with *q*<*q*

cutoff. It is reassuring to observe that the number of sites that are deemed randomized is minimized by phylogenetically plausible circular orderings *?*, Figure 2(A).

Two effects have to be considered. On the one hand, columns with small values of *q*contribute little useful information. On the other hand, a large absolute number of informative sites is necessary to obtain reliable trees. Thus *q*_{cutoff} must not be too large. The most effective values of *q*_{cutoff} also depend on the tree topology. As shown in Figure 2(B) caterpillar trees admit larger improvements in bootstrap support than the balanced trees.

The analysis of artificial data sets suggests a set of simple rules that allow the user to decide under which conditions it makes sense to use noisynoisy to process MSAs prior to using them for phylogenetic reconstruction:

- (1)
If the original alignment already yields trees with very high average bootstrap support, there is nothing to be gained.

- (2)
Data-sets with less than about 10 taxa are unlikely to improve.

- (3)
The best cutoff value for

*q*depends on the tree topology and in particular on the number of taxa. It pays in general to determine the maximum of the gain in some parameter of tree stability as a function of*q*and to use the corresponding optimal cutoff value.

The current release [42] of noisynoisy can process DNA, RNA, and protein sequences.

## Aliscore

The aliscorealiscore approach has been shown to work well in simulations [43],[44], single gene [45]-[49] and multi-gene approaches [50]. However, as for every masking program arbitrary decisions have to be made as well. For example, the sliding window size has to be set by the user. A larger window size makes the algorithm less sensitive to small sections of randomization. A natural minimal window size is 4, below this window size a distinction between random or non-random similarity is not possible.

A big advantage of the approach is that single splits can be directly evaluated. aliscorealiscore offers the possibility to define a split in the MSA from which pairwise comparisons are drawn. It thus offers the possibility to generate a consensus profile for just the split under consideration. This tool can become particularly important, if different outgroup taxa are compared with a set of ingroup species. The best outgroup choice is the set of taxa which minimizes the extent of randomization between outgroup and ingroup. The current release of aliscorealiscore can process DNA, RNA, and protein sequences.

## Quality of a data matrix: MARE

A typical feature of phylogenomic data is the frequent occurrence of missing data in concatenated supermatrices up to the point where more than 80% of the data are missing [51],[52]. The effect of missing data on tree inference is still unclear and it appears that a general rule can not be derived from several simulation and empirical studies [51],[53],[54]. The take-home message of these studies is that data masking, which can increase data saturation, seems advisable. In its simplest form, we are given a bipartite graph *G*=(*X*?*Y*,*E*) describing by its edges {*x*,*y*}?*E* which gene *x*?*X* is present in which species *y*?*Y*. An ideal data set is a maximal biclique, i.e., a maximal complete subgraph of *G*[55],[56]. Since this would lead to the removal of too many genes and taxa, in a relaxed version, one seeks a *quasi-biclique*[57], requiring that each gene is present at least in a prescribed fraction of taxa, and each taxon is represented by a minimum fraction of genes. It is worth noting that the same problem appears in the analysis of protein-protein interaction networks and has received considerable attention in this context [58]. Although the maximum vertex biclique problem is solvable in polynomial time [59], many of its variants [60] and in particular the more relevant quasi-biclique problems are NP-complete [58],[61]. Thus exact algorithms are applicable only to to relatively small data. In addition, earlier methods do not consider differences in the information content of taxa and genes, which might be a major drawback.

## Concluding remarks

Although many studies have been directed at a better understanding of artifacts in phylogeny reconstruction such as long branch attraction or homoplasy [7],[63],[64], we still lack a comprehensive understanding of how biases can be recognized in data sets prior to the estimation of a phylogenetic tree. Instead, often time extensive computational resources are expended to reconstruct phylogenies with disappointing results that can be identified only *a posteriori* as artifacts. It is then problematic at best to distinguish artefactual input data from issues such as inadequate models of evolution.

In this minireview we have briefly discussed first attempts at an *a priori* assessment of different aspects of data quality that aim at the identification of potentially problematic taxa or characters. It is of utmost importance to ensure that such methods do not make any assumptions on phylogenetic relationships, because such implicit information may then inadvertently be enforced in the “data cleaning” step, and thus transmitted to the phylogenetic reconstruction methods.

Despite very encouraging results obtained with tools such as noisynoisy, aliscorealiscore, and maremare, much additional research focused on dissecting confounding signal will be necessary for a comprehensive understanding of analyses artifacts. NoisyNoisy and aliscorealiscore address the decay of phylogenetic signal induced by multiple saturation, the aliscorealiscore algorithm can deal with heterogeneous composition of nucleotide sequences, and maremare indirectly scores the influence of missing data as well. However, all of these approaches do not directly dissect the separate influence of these confounding factors on tree reconstructions. This must be a focus of future work, because substitutional saturation, heterogeneous sequence composition, non-stationary substitution processes, and the non-random distribution of missing data can constitute strong confounding factors, in particular in phylogenomic analyses. It surprising, therefore, that a standard canon of tools to study these effects *a priori* to tree reconstructions is still missing.

## Authors' contributions

BM and PFS wrote the first draft of this review. All authors read and approved the final manuscript.

## Declarations

## Authors’ Affiliations

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